Performa Pertumbuhan dan Ekspresi Gen Hormon Pertumbuhan Sidat (Anguilla bicolor bicolor) Stadia Elver Yang diberi Pakan Mengandung Kromium Pikolinat
Abstract
The administration of chromium picolinate (Cr-Pic) is expected to positively influence the growth of eel (Anguilla bicolor) and the expression of the growth related gene. The purpose of this research was to study the effect of chromium picolinate (Cr-Pic) on the growth performance and expression of growth hormone encoding gene of eel (Anguilla bicolor), a species of fish that is in the process of domestication. The experiment consisted of four treatments: without Cr-Pic (P0), with Cr-Pic of 1 mg / kg of feed (P1), 2 mg / kg of feed (P2), and 3 mg / kg of feed (P3). Eel growth performance was analyzed based on absolute and daily growth rates. Observation of the level of growth hormone (GH) gene expression was performed using real-time RT-PCR method. The results showed that administration of Cr-Pic did not significantly increase eel growth rate. The expression level of growth hormone genes in eel fed 2 and 3 mg Cr-Pic / kg was higher than the expression level of this gene in eel fed without Cr-Pic. The results showed that the level of growth hormone expression did not appear to be related to growth rate of the eel.
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Ahmed, A. R., Jha, A. N., & Davies, S. J. (2012). The Efficacy of Chromium as a Growth Enhancer for Mirror Carp (Cyprinus carpio L): An Integrated Study Using Biochemical, Genetic, and Histological Responses. Biological Trace Element Research, 148(2), 187–197. https://doi.org/10.1007/s12011-012-9354-4
Aryansyah, H., Mokoginta, I., & Jusadi, D. (2007). Growth performance of African catfish (Clarias sp.) juvenile fed on the diets containing various chromium content. Jurnal Akuakultur Indonesia, 6(2), 171–176. https://journal.ipb.ac.id/index.php/jai/article/view/4024
Aslamyah, S., Zainuddin, & Badraeni. (2019). Pengaruh suplementasi ekstrak Lumbricussp. dalam pakan fermentasiterhadap kinerja pertumbuhan, komposisi kimiawi tubuh, dan indeks hepatosomatik ikan bandeng, Chanos chanos Forsskal, 1775. Jurnal Iktiologi Indonesia, 19(2), 271–282.
Bagheri, S., Gholamhosseini, A., & Banaee, M. (2022). Investigation of Different Nutritional Effects of Dietary Chromium in Fish: A Literature Review. Biological Trace Element Research. https://doi.org/10.1007/s12011-022-03326-z
Boyd, C. E., Lim, C., Queiroz, J., Salie, K., de Wet, L., & McNevin, A. (2008). Best Management Practices for Responsible Aquaculture.
Chellappa, S., Huntingford, F. A., Strang, R. H. C., & Thomson, R. Y. (1995). Condition factor and hepatosomatic index as estimates of energy status in male three-spined stickleback. Journal of Fish Biology, 47(5), 775–787. https://doi.org/10.1111/j.1095-8649.1995.tb06002.x
Cui, P., Cheng, Z., & Sun, J. (2022). Effects of different chromium sources on growth performance, serum biochemical, hepatopancreas glycometabolism enzymes activities, IR, GLUT2 and SGLT1 gene expression of common carp (Cyprinus carpio). Aquaculture Research, 53(4), 1573–1581. https://doi.org/https://doi.org/10.1111/are.15692
Fahmi, M. R., & Hirnawati, R. (2010). Keragaman Ikan Sidat Tropis (Anguilla sp.) di Perairan Sungai Cimandiri, Pelabuhan Ratu, Sukabumi. Prosiding Forum Inovasi Teknologi Akuakultur, 1–10.
Fitriadi, M. W., Basuki, F., & Nugroho, R. A. (2014). Pengaruh Pemberian Recombinant Growth Hormone (Rgh) Melalui Metode Oral dengan Interval Waktu yang Berbeda Terhadap Kelulushidupan dan Pertumbuhan Larva Ikan Gurame Var Bastard (Osphronemus gouramy Lac, 1801). Journal of Aquaculture Management and Technology, 3(2), 61–68. https://ejournal3.undip.ac.id/index.php/jamt/ article/view/5065
Forlenza, M., Kaiser, T., Savelkoul, H. F. J., & Wiegertjes, G. F. (2012). The Use of Real-Time Quantitative PCR for the Analysis of Cytokine mRNA Levels. Methods Mol Biol., 820, 7–23. https://doi.org/10.1007/978-1-61779-439-1
Galster, H. (1991). pH Measurement. Fundamentals, Methods, Applications, Instrumentation. Weinheim, Germany: Wiley-VCH.
Giri, A. K., Sahu, N. P., & Dash, G. (2021). Improvement in the growth status and carbohydrate utilization of Labeo rohita (Hamilton, 1822) fingerlings with dietary supplementation of chromium picolinate. Fish Physiology and Biochemistry, 47(2), 599–616. https://doi.org/10.1007/s10695-021-00934-9
Giri, A. K., Sahu, N. P., Saharan, N., & Dash, G. (2014). Effect of dietary supplementation of chromium on growth and biochemical parameters of Labeo rohita (Hamilton) fingerlings. Indian Journal of Fisheries, 61(2), 73–81.
Jönsson, E., & Björnsson, B. T. (2002). Physiological functions of growth hormone in fish with special reference to its influence on behaviour. Fisheries Science, 68(sup1), 742–748. https://doi.org/10.2331/fishsci.68.sup1_742
Li, H., Meng, X., Wan, W., Liu, H., Sun, M., Wang, H., & Wang, J. (2018). Effects of chromium picolinate supplementation on growth, body composition, and biochemical parameters in Nile tilapia Oreochromis niloticus. Fish Physiology and Biochemistry, 44(5), 1265–1274. https://doi.org/10.1007/s10695-018-0514-0
Liu, T., Wen, H., Jiang, M., Yuan, D., Gao, P., Zhao, Y., Wu, F., & Liu, W. (2010). Effect of dietary chromium picolinate on growth performance and blood parameters in grass carp fingerling, Ctenopharyngodon idellus. Fish Physiology and Biochemistry, 36(3), 565–572. https://doi.org/10.1007/s10695-009-9327-5
Lobo, C., Tjendanawangi, A., & Rebhung, F. (2022). Pengaruh penambahan kromium-ragi (Cr Organic) pada pakan terhadap laju pertumbuhan ikan kerapu cantang (Epinephelus fuscogutattus lanceolatus). Jurnal Aquatik, 5(2), 16–23.
Madinawati, Serdiati, N., & Yoel. (2011). Pemberian Pakan yang Berbeda terhadap Pertumbuhan dan Kelangsungan Hidup Benih Ikan Lele Dumbo (Clarias gariepinus). Media Litbang Sulteng, 4(2), 83–87.
Mehrim, A. I. (2012). Effect of Dietary Chromium Picolinate Supplementation on Growth Performance, Carcass Composition and Organs Indices of Nile Tilapia (Oreochromis niloticus L.) Fingerlings. Journal of Fisheries and Aquatic Science, 7(3), 224–232. https://doi.org/10.3923/jfas.2012.224.232
Mukti, R. C., Utomo, N. B. P., & Affandi, R. (2014). Fish oil supplementation in commercial diet on growth of Anguilla bicolor bicolor. Jurnal Akuakultur Indonesia, 13(1), 54–60. https://doi.org/https://doi.org/10.19027/jai.13.54-60
Ozaki, Y., Fukada, H., Tanaka, H., Kagawa, H., Ohta, H., Adachi, S., Hara, A., & Yamauchi, K. (2006). Expression of growth hormone family and growth hormone receptor during early development in the Japanese eel (Anguilla japonica). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 145(1), 27–34. https://doi.org/https://doi.org/10.1016/j.cbpb.2006.05.009
Politis, S. N., Mazurais, D., Servili, A., Zambonino-Infante, J.-L., Miest, J. J., Sorensen, S. R., Tomkiewicz, J., & Butts, I. A. E. (2017). Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae. PloS One, 12(8), e0182726. https://doi.org/10.1371/ journal.pone.0182726
Rachmawati, F. N., Susilo, U., & Muslih. (2017). Karakteristik reproduksi ikan sidat Anguilla bicolor McClelland , 1844 yang diinduksi GNRH-analog. Jurnal Iktiologi Indonesia, 17(2), 155–163.
Rakhmawati, R., Suprayudi, M. A., Setiawati, M., Widanarni, W., Junior, M. Z., & Jusadi, D. (2018). Bioefficacy of dietary chromium picolinate and chromium yeast on growth performance and blood biochemical in Red Tilapia, Oreochromis niloticus (Linnaeus). Aquaculture Research, 49(2), 839–846. https://doi.org/https://doi.org/10.1111/are.13527
Reinecke, M., Bjornsson, B. T., Dickhoff, W. W., McCormick, S. D., Navarro, I., Power, D. M., & Gutierrez, J. (2005). Growth hormone and insulin-like growth factors in fish: where we are and where to go. General and Comparative Endocrinology, 142(1-2), 20–24. https://doi.org/10.1016/j.ygcen.2005.01.016
Ren, M., Mokrani, A., Liang, H., Ji, K., Xie, J., Ge, X., & Liu, B. (2018). Dietary Chromium Picolinate Supplementation Affects Growth, Whole-Body Composition, and Gene Expression Related to Glucose Metabolism and Lipogenesis in Juvenile Blunt Snout Bream, Megalobrama amblycephala. Biological Trace Element Research, 185(1), 205–215. https://doi.org/10.1007/s12011-018-1242-0
Sari, E. P., Mokoginta, I., & Jusadi, D. D. (2019). Pengaruh Pemberian Kromium-Ragi dalam Pakan Terhadap Kinerja Pertumbuhan Ikan Baung (Hemibagrus nemurus Blkr). 1(Furuichi 1988), 17–23.
Setijanto, S., Sulistyo, I., & Eko, B. (2014). Penentuan Waktu Pengambilan Benih dan Diet Ikan Sidat (Anguilla bicolor Mc Clelland) di Sungai Serayu.
Soeprijanto, A., Guntur, G., & Fakhri, M. (2018). Application of Probiotic and Fermented Feed in the Nursery of Anguilla bicolor. Jurnal Perikanan Universitas Gadjah Mada, 20(1), 19–22. https://doi.org/https://doi.org/10.22146/jfs.34207
Sulistyo, I., Fontaine, P., Rinchard, J., Gardeur, J. N., Migaud, H., Capdeville, B., & Kestemont, P. (2000). Reproductive cycle and plasma levels of steroids in male Eurasian perch Perca fluviatilis. Aquatic Living Resources, 13(2), 99–106. https://doi.org/10.1016/S0990-7440(00)00146-7
Syakuri, H., Setiawan, A. C., & Ekasanti, A. (2014). 2014. Prevalensi, Intensitas, dan Variasi Genetik Nematoda Anguillicola yang Menginfeksi Sidat (Anguilla spp.) di Sekitar Pantai Selatan Jawa Tengah. Laporan Fundamental.
Ward, W. J. (2017). Standard Operating Procedure EAP023, Version 2.5, Collection and Analysis of Dissolved Oxygen (Winkler Method). Washington State Department of Ecology. https://apps.ecology.wa.gov/publications/SummaryPages/1703202.html
Weatherley, A. H., & Gill, H. S. (1987). Growth increases produced by bovine growth hormone in grass pickerel, Esox americanus vermiculatus (Le Sueur), and the underlying dynamics of muscle fiber growth. Aquaculture, 65(1), 55–66. https://doi.org/https://doi.org/10.1016/0044-8486(87)90270-5
Xi, G., Xu, Z., Wu, S., & Chen, S. (2001). Effect of Chromium Picolinate on Growth Performance, Carcass Characteristics, Serum Metabolites and Metabolism of Lipid in Pigs. Asian-Australas J Anim Sci, 14(2), 258–262. https://doi.org/10.5713/ajas.2001.258
Yamaguchi, K., Yasuda, A., Kishida, M., Hirano, T., Sano, H., & Kawauchi, H. (1987). Primary structure of Eel (Anguilla japonica) growth hormone. General and Comparative Endocrinology, 66(3), 447–453. https://doi.org/https://doi.org/10.1016/0016-6480(87)90256-5
DOI: http://dx.doi.org/10.33087/akuakultur.v8i1.152
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